7th International Conference Sample Prep 2013 - 第七屆國際試樣製備學會、2013年 -
2013年5月9 - 10日 美國,加州,聖地亞哥,希爾頓聖地牙哥度假村及水療中心

 
         
關於參展
Catalog
Choose your language
Chinese
Japanese
Korean
English


SAMPLE PREP 2013,是Knowledge Foundation在各地召開的Sample Prep會議系列其中之一,是以試樣製備技術的專家為對象的國際高評價的學會。針對生物醫學,生物學,化學等領域的藥物和物質、威脅,在重點照護檢驗和研究室,各種的現場環境進行檢測,是以鑑定,診斷,分析的試樣製備技術為焦點的學會,也介紹研究開發的最新成果,貼近市場的技術,各種用途等。今年的學會預定將涵蓋以下的專題。

- 試樣採集,(事前)濃縮,溶解,標的萃取的紮實方法

- 早期診斷的試樣製備的重要作用

- 從試樣製備到序列分析迅速作業的方法

- 重點照護檢驗的取樣調查,檢測,分析

- 微流體工程所利用的試樣製備

- 晶片試樣製備

- 可靠取樣調查法(試樣製備的自動高通量組合方法)

- 因應高感度的試樣調整的各種放大法

- 各系統及整合模組方法的試樣製備

- 引進生物檢測和取樣調查的技術和設備的可能性高的最終消費者

- 試樣製備和各種應用技術的替代方法和創新方法

- 可現場使用的設備-兼容性/可靠性/擴充性

 

這個學會預定將介紹生物武器的防禦,現場和重點照護檢驗的生物醫學的應用和臨床應用,食品和水的檢驗,在環境和農業領域的取樣調查等可利用的可靠取樣調查和生物司法鑑定的新手法。會期中,將聚集行政機關和大學,企業等最高層級的專家們,針對以技術開發和實用化為焦點的以下問題和領域展開討論。

- 標的濃縮與背景耗竭技術

- 細胞及分子層級的稀釋的物體分離

- 不融和的煉製

- 可萃取有力的(不能過濾)基材的新方法

- 新一代測序的核酸及蛋白質為基礎的試樣製備

- 試樣製備對應的奈米技術和細微化的課題

- 試樣製備,檢測,分析用新化驗和定序技術

- 各種用途的試樣製備的標準化和法規問題

- 因應檢測/診斷和醫藥品的試樣調整技術


 
 
 

媒體贊助商及會議合作夥伴

 
 
2013年5月9日(星期四)
 
8:00        登記手續,展覽會視察/海報發表的準備,咖啡
 
8:50        主辦代表的歡迎和開幕致詞
 
9:00        新一代生物感應器開發對各種試樣製備方法的影響
Charles Young, PhD, Principle Professional Staff, Asymetric Operations Dept, The Johns Hopkins University Applied Physics Laboratory; Assistant Research Professor, Dept of Geography and Environmental Engineering, The Johns Hopkins University
Biological sensors represent one facet of a system designed to identify a biological agent. In addition to the sensor, a detection system must account for sample acquisition, sample storage/transport, sample preparation, assay design/performance and data management. Therefore, a systems approach is critical for the development of next generation sensors since changes to one component can impact the entire system. Over the past 5 years, researchers at JHU/APL have been tracking biosensor development and building a database of commercial-off-the-shelf systems and their capabilities. In this presentation we will discuss the database, current sensor trends and their implication for sample preparation.
 
9:30        重點照護診斷的試樣製備方法
Richard Allen, PhD, Senior Scientist, Biodefense and Food Safety, Luminex Corporation
Complex biological states such as transmissible infectious disease involve a dynamic relationship between pathogenic organisms and their hosts. Accurate assessment of this relationship presents a unique instrumentation and assay challenge. To provide a more comprehensive snapshot of an evolving infection, efforts at Luminex Corporation include development of an automated, field portable diagnostic system based on the open architecture xMAP technology. A key aspect of this system is the ability to test for both the pathogen itself, through molecular techniques, and also host biomarkers that signal the course of infection via affinity capture reagents. Taken together, these data will yield a more accurate and timely treatment, leading to better clinical outcomes than either test alone. The challenge in developing such a system is to devise sample preparation protocols that 1) are versatile enough to encompass either technique, 2) can be deployed onto an automated, inexpensive disposable cartridge, and 3) are rapid enough to both inform clinical decisions and provide multiple measurements throughout disease progression. Luminex will provide an update on sample preparation techniques being investigated for this effort and provide data from initial testing.
 
10:00    試料發送系統的想法
John C. Carrano, PhD, President and CEO, Paratus Diagnostics, LLC
In this paper we posit that in order to realize pragmatic point-of-care medical diagnostic devices capable of meeting the rigorous requirements of CLIA waiver, that one must solve the inherent “impedance mismatch” between the clinical acquisition of a human patient sample and its delivery to the POC Dx system or device. We work from the assumption that specimen acquisition from the patient must follow standard clinical practice, and that the POC system (e.g. “device”) will be designed to follow a yet to be determined international interface standard (similar in principle to the now ubiquitous Luer-Lock standard). We will present a design concept for a “specimen delivery system” and the associated proposed interface standard, along with experimental data from preliminary rapid prototype models.
 
10:30    休息時間,參觀展覽會/海報發表
 
11:00    即時基因組學:從珊瑚研究到臨床應用
Forest L. Rohwer, PhD, Professor of Biology, San Diego State University
Abstract is not available at time of publishing. Please visit www.KnowledgeFoundation.com for the latest Program updates.
 
11:30    病原體生死判斷聚合酵素鏈鎖反應(PCR)法對應的技術
Sarah Fakih, PhD, Senior Scientist, Food Safety Testing R&D, QIAGEN GmbH, Germany
Nucleic acid detection methods, such as real-time PCR, provide fast and powerful tools to analyze samples for the presence of potentially harmful microbes, but also hold the risk of false positives by detecting nucleic acid from harmless dead cells. We addressed this by a universally applicable tool box for an advanced viability PCR, which centers on the DNA-masking compound Propidium monoazide (PMA), suppressing amplification signals from dead cell DNA. We have pre-developed viability PCR as a complete standardized system to be used with a new illumination device designed to catalyze the PMA reaction. The new tool box system, the illumination device and its function together in viability PCR applications are presented with multiple sets of user data.
 
12:00    重點照護檢驗環境對應的穩健自我完成型試樣調整盒
Season Wong, PhD, Co-Founder and Director, AI Biosciences, Inc.
This presentation will cover the development of AI Biosciences Inc.’s Sample Preparation Cartridge (SPC) which delivers high quality nucleic acids for point-of-care molecular analysis. Our SPC can be operated manually or hands-free using battery pack. The SPC is a closed-system that eliminates cross-contamination and provides hassle-free waste disposal. We will demonstrate its utilities using a wide range of samples. The detection of the derived nucleic acid in a non-laboratory setting will also be discussed.
 
12:30    Knowledge Foundation會員計劃提供的午餐會
 
2:00        慢性淋巴性白血病(CLL)患者血液的循環細胞自由DNA的分離與檢測-「從試樣製備到序列分析作業迅速化」
Michael J. Heller, PhD, Professor, Depts of Bioengineering and Nanoengineering, University of California San Diego*
We have developed a unique sample to answer dielectrophoretic (DEP) technology for rapid isolation and detection of cancer related cell free circulating DNA (cfc-DNA) biomarkers, bacteria and virus directly form blood and other complex biological samples. Using this DEP microarray device, cfc-DNA from chronic lymphocytic leukemia (CLL) patients could be isolated directly from 50uls of unprocessed whole blood in about 15 minutes. The cfc-DNA was then eluted from the DEP chip, PCR amplified using CLL specific primers and sequenced to determine CLL patient specific polymorphisms and mutations. Overall, the use of DEP devices for the rapid isolation of cfc-DNA from a small volume of blood will allow “noninvasive liquid biopsy” point of care (POC) detection and diagnosis of incipient, residual, and recurrent cancer and other diseases.
*In collaboration with: Jennifer Marciniak and Avery Sonnenberg
 
2:30        檢測環境中的生物學因子的一般試樣製備方法
Martin McDonnell, PhD, Principal Scientist, Detection Department, Defense Science and Technology Laboratory - DSTL Porton Down, United Kingdom
Abstract is not available at time of publishing. Please visit www.KnowledgeFoundation.com for the latest Program updates.
 
3:00        迅速從血液調整細菌試樣的方法
Alexis Sauer-Budge, PhD, Senior Research Scientist, Fraunhofer Center for Manufacturing Innovation, Fraunhofer USA
Traditionally, bacterial pathogens in the blood have been identified using culture-based methods that can take several days to obtain results. This can lead to physicians making treatment decisions based on an incomplete diagnosis contributing to patient morbidity. To decrease diagnosis time, we are developing a novel sample preparation device for isolating and concentrating dilute bacteria from blood. The device is designed to be a single-use disposable that can be used manually or with a fully automated instrument.
 
3:30        休息時間,參觀展覽會/海報發表
 
4:00        藉由Raman光譜的水中微生物污染無試劑的迅速鑑定方法
Gregory Auner, PhD, Professor, Electrical and Computer Engineering, SSIM Director, Wayne State University
We are developing a reliable and rapid method to assess microbial contamination in water. Raman Spectroscopy is a reagentless, non-destructive, technique that provides a unique spectral fingerprint of bacteria without sample preparation and is conducive to field application since it can provide both qualitative and quantitative analysis. We will present Raman identification of E. coli, Salmonella, Rahnella aquatilis, Enterobacter, Vibrio fluvialis, Pseudomonas, Bacillus subtilis, Listeria, Staphylococcus, and Streptococcus.
 
4:30        樹狀聚合物修飾的多層奈米碳管為基礎的人體細胞傳染性蛋白質的無標記電化學檢測
Hafsa Korri-Youssoufi, PhD, Researcher, Institut de Chimie Moléculaire et des Matériaux d’Orsay â€g ICMMO, University Paris-Sud, France
The present work aims to develop an electrochemical aptamer based biosensor able to detect human cellular prions PrPC as a model biomarker of prion disease with high sensitivity. We designed biosensor using multiwalled carbon nanotubes (MWCNTs) modified with polyamidoamine dendrimers (PAMAM) of fourth generation (G4) able by their amino group to attach ferrocenyl redox marker and the DNA aptamer as bioreceptor. MWCNTs, thanks to their nanostructure organization and electrical properties, allow the distribution of aptamer and redox markers over the electrode surface. We demonstrated that the interaction between aptamer and prion protein leads to variation in electrochemical signal of the ferrocenyl group. High sensitivity detection limit of 0.5 pM and wide linear range of detection from 1 pM to 10 µM has been demonstrated. Detection of PrPC in spiked blood plasma has been achieved and demonstrated a recovery of 85%.
 
5:00        匣式重點照護檢驗HIV病毒數量檢驗設備對應的No-Spin試樣製備技術
Shan Gao, PhD, Senior Chemical Engineer, Wave 80 Biosciences, Inc.*
Porous polymer monoliths (PPMs) were developed to prepare nucleic acids from HIV virion-spiked whole blood or plasma samples for quantification of viral RNA using both standard qRT-PCR assays and a novel high-sensitivity bipartite signal amplification assay (BSAA). The efficiency of optimized in-cartridge, glycogen-mediated and monolith-based extraction of viral RNA was approximately 50%. This is comparable to standard centrifuge-based methods involving either phenol-chloroform extraction s or commercial spin columns. The new sample preparation method is particularly well suited for the development of point-of-care HIV viral load testing systems with sophisticated functionality and low cost.
*In collaboration with: F.Wang, H.Negussie, J.Frey, A.Arsham, A.Droitcour, L.Mazzola, D.Laser, Wave 80 Biosciences; and A.Fan, C.Klapperich, Boston University
 
5:30        參展商/贊助商的簡報
 
6:00        第1天結束
 
 
 
2013年5月10日(星期五)
 
8:00        參觀展覽會/海報發表,咖啡
 
9:00        自我完成型體外診斷盒
David Wright, CEO, Wi Medical Device Development, Inc.
This talk is aimed at the methods of designing a fully integrated self-contained IVD cartridge. The evolving world of complex IVD test cartridges is moving towards a self-contained format, where the fluidic assay is contained, from sample pre to waist containment. The need for this format is based on taking complex assays from the lab into the field, where persons of moderate skills may execute a test with confidence, in a short period of time, at acceptable cost. The world of diagnostics is changing the world of applied therapy, and thusly, the cost of healthcare.
 
9:30        從臨床試樣直接檢測萊姆病伯氏疏螺旋體分子的改良試樣製備及標的濃縮技術
Mark W. Eshoo, PhD, Director of New Technology Development, Ibis Biosciences Inc., an Abbott Company
Abstract is not available at time of publishing. Please visit www.KnowledgeFoundation.com for the latest Program updates.
 
10:00    微機電(MEMS)-微流體工程的整合標準設計:磁為基礎的試樣濃縮/檢測技術範例
Mark Tondra, PhD, President, Diagnostic Biosensors
Commercialization of Point of Care (POC) and integrated Lab on a Chip (LOC) products is advancing. One enabling factor is industry standards for microfluidic interfaces between two devices, like a detector chip and a concentrator chip. These standards facilitate the design of higher-level micro-scale systems using plug-and-play architectures. The state-of the art in MEMS-Microfluidics industry standards will be presented. Our own use of these standards in design of multi-tube fluidics interfaces in our biosensor products will be shown, as will applications of devices using magnetic microbeads for sample concentration and manipulation in microfluidic LOC systems.
 
10:30    休息時間,參觀展示會/海報發表
 
11:00    LC-ESI MS/MS分析法的快速的病原體鑑定和抗性預測的完全自動化試樣製備
Patrick Broyer, PhD, Senior Scientist, bioMérieux SA, France
This talk will present a new automated sample prep solution for extraction of proteins and peptides dedicated to LC-ESI-MS analysis. Protocol simplifications, new filtration device for urine and blood & automation of peptide digestion will be described to show the ability of this solution to move to a long 18h protocol to a short 1h fully automated protocol. Results will be showed demonstrating the capabilities to correctly identify resistant microorganisms from positive blood culture. The sample preparation automated prototype is already used in our laboratory providing within 1 hour peptides solution ready to be analyzed by LC-ESI-MS by batches of 30 samples per run.
 
11:30    在複雜環境矩陣進行生物學粒子的辨別和濃縮的新薄膜為基礎的系統開發
Andy Page, President & CTO, InnovaPrep LLC
InnovaPrep LLC will present an update on development of a novel, integratable, membrane-based fractionation and concentration system, under a current Department of Homeland Security Phase II SBIR. Powerful, automated sample preparation techniques are needed for improved detection and identification of rare biological particles in complex environmental samples by autonomous biodetection systems. A novel cartridge containing a series of sharp-cut membrane filters in order of decreasing pore size, along with integrated pneumatic valves and fluid paths, are used to rapidly separate particles present in up to 20 mL of environmental sample by size into separate fractions containing large environmental particles, bacteria, viruses and DNA, and proteins. Each captured fraction is then eluted into separate concentrated volumes of less than 200 µL. Program goals, hurdles, and performance data will be presented.
 
12:00    新一代測序的自動化試樣製備及分析技術
Numrin Thaitrong, PhD, Researcher, National Center for Genetic Engineering and Biotechnology / Sandia National Labs
Thorough characterization of next-generation sequencing library is a required process in the sample preparation workflow. However, this built-in quality control feature is currently not available for small-scale library construction platforms. We have developed an integrated droplet-based digital microfluidics (DMF) architecture with capillary interface to handle sub-microliter sample processing and analysis. The platform providing size distribution and quantization of the library can be coupled to the existing modular DMF “hub” to automate start-to-finish library construction.
 
12:30    午餐
 
2:00        臨床試樣的收集,搬送,調整改善
Marek Smieja, MD, PhD, Associate Professor, Dept of Pathology and Molecular Medicine, McMaster University, Canada*
The development of point of care diagnostic tests for common infectious diseases needs parallel improvements in the ease of collection of clinical samples, and appropriate transport media for in-tube sample stabilization and/or extraction. We studied the effectiveness and reproducibility of Copan’s FLOQSwabs™ for sample collection for respiratory, gastrointestinal, and genitourinary diseases. We determined the feasibility and performance characteristics of self-collected, less-invasive diagnostic samples; evaluated the robustness of transport media (dry swabs, UTM, eNAT, ESwab, MSwab, Cymol) under differing temperature and time conditions; and determined the performance characteristics of various media for antigen-based and molecular diagnostic tests.
*In collaboration with: Copan Diagnostics
 

2:30 Integrated PureLyse® Sample Preparation
Bruce Irvine, CTO, Claremont BioSolutions LLC
Claremont BioSolutions has created a novel method of rapid sample preparation that is entirely disposable. It provides mechanical cell lysis and simultaneously extracts nucleic acids or proteins. Nucleic acid can be extracted in three to five minutes with two to three steps. The disposable PureLyse® device is comprised of a micro-motor and vane that agitate particles at unusually high shear forces. As cells are lysed, nucleic acid binds to the lysing particles. This system delivers high yield RNA preparation as well. This system is also used to very effectively homogenize tissue. The miniature disposable nature of this flow-through cartridge lends itself to integration. Our flagship approach to integration is the OmniValveâ„¢ fluidic system, where we have embedded the PureLyse® chamber within a valve with up to six ports connecting the lysis and extraction cartridge to other chambers. This facilitates integration of DNA extraction with sample introduction, pre-filters, wash, elution, and amplification. This approach was very successfully applied to an NIH funded SBIR project developing a semi-integrated system for detecting Clostridium difficile, resulting in 100 % specificity and 96% sensitivity as compared to standard enzyme immunoassay of stool samples.

 
3:00        全血中的穩定的遺傳基因表達:人體血液試樣的常溫處理的高品質RNA淨化
Vasco Liberal, PhD, Scientist, Biomatrica, Inc.
Expression profiles from blood samples are increasingly used to diagnose, monitor and treat diseases, requiring reliable RNA preservation during sample collection, transport and storage. Transcription profiles can change rapidly, potentially dictating inadequate treatment. Biomatrica has developed a blood collection device that stabilizes RNA in blood cells during ambient temperature sample handling and storage. With its coupled RNA purification kit, it provides a complete solution from blood collection to RNA purification, achieving high yields and great RNA quality, with unaltered gene expression for 14 days of room temperature storage, which can prove of extreme value for better patient treatment.
 
3:30        介紹參展商和贊助商的簡報/出色的海報發表
 
4:00        閉會致詞,學會結束

 


這個學會將進行企業和政府機關,大學等研究人員的海報發表。希望進行海報發表的人員,請把刊載學會資料的1頁摘要(8.5英吋x 11英吋,頁邊1英吋)在2013年4月5日之前以電子郵件傳送。此外,海報發表的申請受理到4月20日,但可能不包含在會議資料中。

海報板的大小:
寬4英呎 x 高3英呎
(海報板也可以是直立式,這個情況下將變成寬3英呎 x 高4英呎 (90cm x 120cm))

注意事項:海報發表申請,請務必要登記參加學會,在事前支付登記費及海報板預約費的時間點上完成海報板發表空間的預約。
 
展覽會的參展

這個學會將為您提供參展與贊助商相關的各種選擇。作為獲得好評的高價值選擇,如下列事項。想瀏覽記載可利用的全部選擇的清單時,請點擊這個網頁的上部組塊的參展相關的PDF鏈接,或詢問本公司。

10x10英呎的展位 費用:2,499美元

包裝的內容:
  • 鄰近整個會議的會場的10x10英呎的展位
  • 全部會議通行證1張
  • 只有展覽會場入場的展位主管人員用通行證1張 (如果需要額外通行證,1張銷售149美元)
  • 學會資料1份
  • 在學會資料活頁夾放入8.5 x 11英吋的黑白廣告(支付199美元的額外費用就可以變更為彩色廣告)
  • 學會結束後發送1次性的參加者的地址標籤1份
  • 貴公司客戶及有潛力客戶的共同郵寄廣告
  • 學會網站的參展商章節刊載參展企業的清單與標誌(附加貴公司網站與學會網站的互相鏈接)
  • 主要議程的「Product Showcase(產品櫥窗)」的15分鐘簡報
  • 刊載會議參展產品和服務相關資料的貴公司網站的鏈接將刊載在會議的電子報上
  • 在主要會議的手冊上刊載參展商 (只限定在期限內申請的參展商)


Discount Accommodations and Travel:
A block of rooms has been allocated at the conference hotel. Please make your reservations by April 3, 2013 to obtain this rate. When making reservations, please refer to the Knowledge Foundation. Contact The Knowledge Foundation if you require assistance.

CONFERENCE VENUE:
San Diego Hilton & Resort
1775 East Mission Bay Drive
San Diego, CA  92109




Hertz is the Official Car Rental Provider for the Knowledge Foundation, Click Below to Make Your Car Reservation


產業會議彙總曆
免費電子郵件通知服務
免費電子郵件通知服務
 
中文官方網站營運:The Knowledge Foundation代理商 日商環球訊息有限公司